Composite

Part:BBa_I13413

Designed by: jkm   Group: iGEM04_MIT   (2005-05-23)


Pbad.RBS.CFP (insertion site) RBS.YFP

Intermediate in building second version of screening plasmid.

Pbad-RBS-CFP-HindIII-NcoI-RBS-YFP


Usage and Biology

Has a deletion at the end of Pbad (which seems to be in the original part). This is after the transcription start and before the translation start, so it shouldn't be significant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//classic/composite/uncategorized
Parameters
None